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(12) UIllt€d States Patent (10) Patent No.: US 8,153,064 B2 Doebler, II et al. (45) Date of Patent: Apr. 10, 2012 (54) SYSTEMS AND DEVICES FOR ISOTHERMAL (58) Field of Classification Search ...................... .. None BIOCHEMICAL REACTIONS AND/OR See application file for complete search history. ANALYSIS (56) References Cited (76) Inventors: Robert W. Doebler, II, Upland, CA (US); Christopher Cooney, Severn, MD U.S. PATENT DOCUMENTS Anna Hickerson, Altadena, A et al‘ (US); James D. Sterling, Upland, CA 4,683,202 A 7/1987 Mullis (US); Ali Nadim, San Marino, CA (US) 4,800,159 A 1/1989 Mullis et al. 5,229,580 A * 7/1993 Chioniere ................... .. 219/521 ~ . ~ ~ ~ ~ 5,315,993 A * 5/1994 Alcala .............. .. 422/82.07 ( * ) Not1ce. SUDJBCF to any d1scla1mer, the term ofth1s 5,464,773 A U/1995 Melendez et al‘ ““““ H 435/3061 Patent 15 extended or adlusted under 35 5,475,610 A * 12/1995 Atwood et a1, ,,,,,,,,,,,,, N 700/269 U-S-C 154(b)by 579 dayS- 5,824,517 A 10/1998 C1el1Z1ateta1. C ' d (21) Appl. NO.Z 12/052,950 ( Ommue ) FOREIGN PATENT DOCUMENTS (22) Filed: Mar. 21, 2008 EP 1 767 630 3/2007 (65) Prior Publication Data (Continued) US 2008/0229849 A1 Sep. 25, 2008 OTHER PUBLICATIONS Related U_s_ Application Data Belgrader et al., “A Minisonicator to Rapidly Disrupt Bacterial
_ _ _ _ Spores for DNA Analysis,” Anal. Chem. 71:4232-4236, 1999. (60) Prov1s1onal application No. 60/896,452, filed on Mar.
22, 2007. (Continued)
(51) Int_ CL Primary Examiner * Jill Warden
G01N 15/06 (200601) Assistant Examiner * Charles D Hammond
G01N 33/00 (2()()6_01) (74) Attorney, Agent, or Firm 4 Seed IP Law Group PLLC
G01N 33/48 (2006.01)
G01N 22/00 (2006.01) (57) ABSTRACT
G01N 21/64 (2006-01) An isothermal reaction and analysis system may include a
G01N21/66 (2006-01) receiver to receive sample holders, a thermal control sub-
G01N 21/ 75 (2006-01) system to control a temperature of the receiver, an excitation
G01N 31/22 (2006-01) subsystem, a detection subsystem and an analysis subsystem.
G01N 33/52 (2006-01) Excitation sources and/or detectors are positioned to enhance
G01N21/76 (2006-01) data collection. Sample holders may include filters, selec-
G01N 1/24 (2006-01) tively blocking and passing wavelengths or bands of electro-
B01L 9/00 (2006-01) magnetic radiation.
(52) U.S. Cl. ............... .. 422/82.08; 422/68.1; 422/82.05;
422/400; 422/561; 436/172; 73/863.11 18 Claims, 3 Drawing Sheets
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Doebler et al., “System, Apparatus and Method for Lysing” U.S. Appl. No. 61/020,072, filed Jan. 9, 2008, 62 pages.
Doebler et al., “System, Apparatus and Method for Material Preparation and/or Handling” U.S. Appl. No. 12/732,070, Preliminary Amendment, filed Mar. 29, 2010, 3 pages.
Doebler et al., “Continuous-Flow, Rapid Lysis Devices for Biodefense Nucleic Acid Diagnostic Systems,” JALA 14:119-125, 2009.
Doebler et al., “Effect of Triton X-100 on Nicking Endonuclease Activity of N.BstNBI during Isothermal Exponential Amplification of Oligonucleotides Performed Using a Hand-held Real-time Fluorescence-based Device,” Keck Graduate Institute, Poster presented in 2005, 1 page.
Farmer et al., “Hand-held thermal-regulating fluorometer,” Review of Scientific Instruments 76(115102):1-5, 2005.
International Search Report for International Application No. PCT/ US2009/030622, mailed Aug. 27, 2009, 4 pages.
International Search Report for International Application No. PCT/ US2010/039872, mailed Mar. 23, 2011, 6 pages.
Irvine et al., “Capture and Elution of Bio-Analytes Via Beads That Are Used to Disrupt Specimens” U.S. Appl. No. 61/220,984, filed Jun. 26, 2009, 67 pages.
Irvine et al., “Capture and Elution of Bio-Analytes Via Beads That Are Used to Disrupt Specimens” U.S. Appl. No. 61/317,604, filed Mar.25, 2010,91 pages.
Kim et al., “Cell lysis on amicrofluidic CD (compact disc),” Lab Chip 4:516-522, 2004.
Paul et al., “A Multichannel Handheld Sensor for Microbial Contaminants,” CICEET Progress Report for the period Mar. 16, 2006 through Sep. 15, 2006, URL:http://ciceet/unh.edu/progressreports/ 2006/9i2006/paul05/, download date Nov. 1, 2011, 3 pages.
US Digital Designs, DNA Amplifier, URL:http://usdd.com/DisplayiPage.php?pageTitle:DNA%20Amplifier, download date Nov. 1, 2011, 1 page.
Written Opinion for International Application No. PCT/US2009/ 030622, mailed Aug. 27, 2009, 4 pages.
Written Opinion for International Application No. PCT/US2010/ 039872, mailed Mar. 23, 2011, 4 pages.
U.S. Patent Apr. 10,2012 Sheet 2 613 US 8,153,064 B2
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