WO2000055339A3 - Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field - Google Patents

Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field Download PDF

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Publication number
WO2000055339A3
WO2000055339A3 PCT/HU2000/000021 HU0000021W WO0055339A3 WO 2000055339 A3 WO2000055339 A3 WO 2000055339A3 HU 0000021 W HU0000021 W HU 0000021W WO 0055339 A3 WO0055339 A3 WO 0055339A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
field
immobilization
high frequency
higher plants
Prior art date
Application number
PCT/HU2000/000021
Other languages
French (fr)
Other versions
WO2000055339A2 (en
Inventor
Zsolt Ponya
Beata Barnabas
Peter Finy
Original Assignee
Mta Mezogazdasagi Ki
Zsolt Ponya
Beata Barnabas
Peter Finy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mta Mezogazdasagi Ki, Zsolt Ponya, Beata Barnabas, Peter Finy filed Critical Mta Mezogazdasagi Ki
Priority to AU34480/00A priority Critical patent/AU3448000A/en
Publication of WO2000055339A2 publication Critical patent/WO2000055339A2/en
Publication of WO2000055339A3 publication Critical patent/WO2000055339A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Abstract

Disclosed herein is a method and a device for the immobilization of polarizable cells of higher plants by the use of a high frequency AC-field wherein the surfaces of the cells are polarized by the use of the AC-field and the cells of polarized surface are then contacted with the surface of an electrode under potential. The invention further concerns simple and highly effective microinjection processes performed by the use of the method and device of the invention, which processes are useful for introducing exogenous DNA into different plant cells, e.g. egg cells/zygotes of wheat.
PCT/HU2000/000021 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field WO2000055339A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU34480/00A AU3448000A (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU9900603A HUP9900603A1 (en) 1999-03-12 1999-03-12 Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current
HUP9900603 1999-03-12

Publications (2)

Publication Number Publication Date
WO2000055339A2 WO2000055339A2 (en) 2000-09-21
WO2000055339A3 true WO2000055339A3 (en) 2000-12-28

Family

ID=89997928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2000/000021 WO2000055339A2 (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field

Country Status (3)

Country Link
AU (1) AU3448000A (en)
HU (1) HUP9900603A1 (en)
WO (1) WO2000055339A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
FR2969497B1 (en) * 2010-12-27 2013-06-28 Ceva Sante Animale LUMINESCENT COMPOSITION AS A BIOMARKER IN AN AVIAN EGG, CORRESPONDING DEVICE AND METHOD.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304486A (en) * 1987-10-09 1994-04-19 Baylor College Of Medicine Method of and apparatus for cell portion and cell fusion using radiofrequency electrical pulse
WO1998001575A1 (en) * 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5723324A (en) * 1995-10-12 1998-03-03 The University Of Akron Apparatus and method for electrostatic endothelial cell seeding and DNA transfection in a vascular prosthesis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304486A (en) * 1987-10-09 1994-04-19 Baylor College Of Medicine Method of and apparatus for cell portion and cell fusion using radiofrequency electrical pulse
US5723324A (en) * 1995-10-12 1998-03-03 The University Of Akron Apparatus and method for electrostatic endothelial cell seeding and DNA transfection in a vascular prosthesis
WO1998001575A1 (en) * 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AKIRA MIZUNO ET AL: "OPT-ELECTROSTATIC MICRO-MANIPULATION OF PROTOPLASTS AND FINE PARTICLES", CONFERENCE RECORD OF THE INDUSTRY APPLICATIONS SOCIETY ANNUAL MEETING,US,NEW YORK, IEEE, vol. MEETING 25, 7 October 1990 (1990-10-07), pages 728 - 733, XP000204120, ISBN: 0-87942-553-9 *
PONYA ET AL.: "Optimisation of intoducing foreign genes into egg cells and zygotes of wheat (Triticum aestivum L.) via microinjection", PROTOPLASMA, vol. 208, 1999, pages 163 - 172, XP000952283 *

Also Published As

Publication number Publication date
WO2000055339A2 (en) 2000-09-21
AU3448000A (en) 2000-10-04
HU9900603D0 (en) 1999-05-28
HUP9900603A1 (en) 2000-12-28

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