DE3856549T2 - Apparat zur Zellpermeabilisierung und Zellfusion unter Verwendung elektrischer Radiofrequenz-Impulse - Google Patents

Apparat zur Zellpermeabilisierung und Zellfusion unter Verwendung elektrischer Radiofrequenz-Impulse

Info

Publication number
DE3856549T2
DE3856549T2 DE3856549T DE3856549T DE3856549T2 DE 3856549 T2 DE3856549 T2 DE 3856549T2 DE 3856549 T DE3856549 T DE 3856549T DE 3856549 T DE3856549 T DE 3856549T DE 3856549 T2 DE3856549 T2 DE 3856549T2
Authority
DE
Germany
Prior art keywords
fusion
radio frequency
frequency pulses
cell permeabilization
electrical radio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3856549T
Other languages
English (en)
Other versions
DE3856549D1 (de
Inventor
Donald C Chang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baylor College of Medicine
Original Assignee
Baylor College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/106,282 external-priority patent/US4822470A/en
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Application granted granted Critical
Publication of DE3856549D1 publication Critical patent/DE3856549D1/de
Publication of DE3856549T2 publication Critical patent/DE3856549T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
DE3856549T 1987-10-09 1988-10-05 Apparat zur Zellpermeabilisierung und Zellfusion unter Verwendung elektrischer Radiofrequenz-Impulse Expired - Fee Related DE3856549T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/106,282 US4822470A (en) 1987-10-09 1987-10-09 Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
US07/238,670 US4970154A (en) 1987-10-09 1988-08-30 Method for inserting foreign genes into cells using pulsed radiofrequency

Publications (2)

Publication Number Publication Date
DE3856549D1 DE3856549D1 (de) 2003-01-30
DE3856549T2 true DE3856549T2 (de) 2003-11-20

Family

ID=26803507

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3855330T Expired - Fee Related DE3855330T2 (de) 1987-10-09 1988-10-05 Verfahren zur zellpermeabilisierung und zellfusion unter verwendung elektrischer radiofrequenz impulse
DE3856549T Expired - Fee Related DE3856549T2 (de) 1987-10-09 1988-10-05 Apparat zur Zellpermeabilisierung und Zellfusion unter Verwendung elektrischer Radiofrequenz-Impulse

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE3855330T Expired - Fee Related DE3855330T2 (de) 1987-10-09 1988-10-05 Verfahren zur zellpermeabilisierung und zellfusion unter verwendung elektrischer radiofrequenz impulse

Country Status (4)

Country Link
US (2) US4970154A (de)
EP (1) EP0710718B1 (de)
AU (1) AU2787989A (de)
DE (2) DE3855330T2 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202013005125U1 (de) * 2013-06-04 2014-09-05 Hugo Vogelsang Maschinenbau Gmbh Vorrichtung zur elektrischen Desintegration von Zellverbänden
DE102018105931A1 (de) * 2018-03-14 2019-09-19 Technische Universität Darmstadt Mikrowellenporator

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141131A (en) * 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
US5149655A (en) * 1990-06-21 1992-09-22 Agracetus, Inc. Apparatus for genetic transformation
JP3104995B2 (ja) * 1991-01-31 2000-10-30 ワーナー−ランバート・コンパニー 抗炎症剤として有用な４，６−ジ−第三ブチル−５−ヒドロキシ−１，３−ピリミジンの置換されたヘテロアリール類似体
WO1993009838A1 (en) * 1991-11-15 1993-05-27 Brigham And Women's Hospital Apparatus and method for long term electrical stimulation of heart muscle cells
US6348327B1 (en) 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5501662A (en) * 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US6132419A (en) * 1992-05-22 2000-10-17 Genetronics, Inc. Electroporetic gene and drug therapy
EP1466968A3 (de) * 1993-03-23 2006-10-04 Cbr Laboratories, Inc. Verfahren und Vorrichtung zur Einkapselung von biologisch aktiven Substanzen
US5422272A (en) * 1993-07-14 1995-06-06 Andrew A. Papp Improvements to apparatus and method for electroporation
JP3423041B2 (ja) * 1993-10-07 2003-07-07 三洋電機株式会社 神経モデル素子
IL108775A (en) * 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
US5795755A (en) * 1994-07-05 1998-08-18 Lemelson; Jerome H. Method of implanting living cells by laser poration at selected sites
US5720921A (en) * 1995-03-10 1998-02-24 Entremed, Inc. Flow electroporation chamber and method
US6074605A (en) * 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
GB9507125D0 (en) * 1995-04-06 1995-05-31 Scient Generics Ltd Travelling wave electrodes
US20060024359A1 (en) * 1995-06-07 2006-02-02 Walker Jeffrey P Drug delivery system and method
US6041252A (en) * 1995-06-07 2000-03-21 Ichor Medical Systems Inc. Drug delivery system and method
JPH0994288A (ja) * 1995-09-28 1997-04-08 Rimoderingu Touentei One:Kk 微生物の不活化・破壊方法
CA2227296C (en) 1995-10-11 2003-12-23 Regeneration Technology Bio-active frequency generator and method
US5891182A (en) 1995-10-11 1999-04-06 Regeneration Tech Bio-active frequency generator and method
US6010613A (en) * 1995-12-08 2000-01-04 Cyto Pulse Sciences, Inc. Method of treating materials with pulsed electrical fields
US5854039A (en) * 1996-07-17 1998-12-29 Zymogenetics, Inc. Transformation of pichia methanolica
EP1007732B1 (de) 1997-01-17 2006-07-26 Maxygen, Inc. EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
CN1109101C (zh) * 1997-06-10 2003-05-21 塞托·帕尔斯科技公司 处理带有可变方向的电场的物质的方法和装置
EP1329502A3 (de) * 1997-06-10 2004-02-11 Cyto Pulse Sciences, Inc. Verfahren und Apparatur zur Behandlung von Material mit elektrischen Feldern unterschiedlicher Orientierung
WO1998056893A1 (en) * 1997-06-10 1998-12-17 Walters Richard E Method and apparatus for treating materials with electrical fields having varying orientations
US6117660A (en) * 1997-06-10 2000-09-12 Cytopulse Sciences, Inc. Method and apparatus for treating materials with electrical fields having varying orientations
ATE275423T1 (de) 1997-06-30 2004-09-15 Aventis Pharma Sa Verabreichung der nukleinsäure in den quergestreiften muskel
IL133708A0 (en) 1997-06-30 2001-04-30 Rhone Poulence Rorer S A Improved method for transferring nucleic acid into multicelled eukaryotic organism cells and combination thereof
USH2119H1 (en) 1997-11-17 2005-06-07 The United States Of America As Represented By The Secretary Of The Navy Acoustic fusion of aquatic animal tissue cells with biological agents
DE19752961C1 (de) * 1997-11-28 1999-07-15 Fraunhofer Ges Forschung Verfahren und Vorrichtung zum Aufschluß biologischer Zellen zur Extraktion und Analyse der Zellinhalte
GB9804246D0 (en) * 1998-02-27 1998-04-22 Babraham The Inst Electropermeabilisation method and apparatus
US7157083B2 (en) * 1998-04-17 2007-01-02 Surrogate Pharmaceutical Pathways, Llc Compositions and methods for treating retroviral infections
DE19823047C1 (de) 1998-05-22 1999-08-26 Fuhr Verfahren und Vorrichtung zur Permeation biologischer Objekte
US6027488A (en) * 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
US5973119A (en) * 1998-06-05 1999-10-26 Amgen Inc. Cyclin E genes and proteins
ATE491780T1 (de) * 1998-06-10 2011-01-15 Univ South Florida Elektrofusions-kammer
US6344541B1 (en) * 1998-09-25 2002-02-05 Amgen Inc. DKR polypeptides
AU2150300A (en) * 1998-12-07 2000-06-26 Acacia Biosciences Inc. Multi-channel electrode arrays
US6314316B1 (en) * 1998-12-17 2001-11-06 University Of South Florida Nonpenetrating electroporation device and method
US6937890B2 (en) * 1998-12-17 2005-08-30 University Of South Florida Nonpenetrating electroporation device
DE19859459A1 (de) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Mikrosysteme zur Zellpermeation und Zellfusion
US8624010B1 (en) 1999-02-03 2014-01-07 Steven K. Yoshinaga Nucleic acids encoding B7RP1
US7435796B1 (en) 1999-02-03 2008-10-14 Amgen Inc. Antibodies which bind B7RP1
US7708993B2 (en) * 1999-02-03 2010-05-04 Amgen Inc. Polypeptides involved in immune response
JP5000804B2 (ja) 1999-02-03 2012-08-15 アムジエン・インコーポレーテツド 免疫応答に関与する新規ポリペプチド
US6146599A (en) 1999-02-24 2000-11-14 Seagate Technology Llc Dielectric barrier discharge system and method for decomposing hazardous compounds in fluids
HUP9900603A1 (hu) * 1999-03-12 2000-12-28 MTA Mezőgazdasági Kutatóintézete Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával
US6678558B1 (en) * 1999-03-25 2004-01-13 Genetronics, Inc. Method and apparatus for reducing electroporation-mediated muscle reaction and pain response
WO2000058479A1 (en) 1999-03-26 2000-10-05 Amgen Inc. Beta secretase genes and polypeptides
US6352838B1 (en) 1999-04-07 2002-03-05 The Regents Of The Universtiy Of California Microfluidic DNA sample preparation method and device
FR2792207B1 (fr) * 1999-04-15 2001-06-08 Electricite De France Procede de traitement d'un flux aqueux par electropulsation a champ parallele a l'ecoulement, chambre de pulsation et applications
SE9902817D0 (sv) 1999-07-30 1999-07-30 A & Science Invest Ab A method for selective electrofusion of at least two fusion partners having cell-like membranes
US7459540B1 (en) * 1999-09-07 2008-12-02 Amgen Inc. Fibroblast growth factor-like polypeptides
US7408047B1 (en) * 1999-09-07 2008-08-05 Amgen Inc. Fibroblast growth factor-like polypeptides
US6900043B1 (en) * 1999-09-21 2005-05-31 Amgen Inc. Phosphatases which activate map kinase pathways
US7198702B1 (en) * 1999-09-30 2007-04-03 Wako Pure Chemical Industries, Ltd. Method for separating substances using dielectrophoretic forces
WO2001051625A2 (en) * 2000-01-14 2001-07-19 Mycoferm Technologies Inc. Apparatus and method for enhanced biomass production with electrical waveform shaping
EP1254951B1 (de) * 2000-01-27 2011-01-12 The Mollennium Laboratories Molekültransferierende vorrichtung, zusatz für molekültransferierende vorrrichtung und molekül-transferierendes verfahren
US20050239061A1 (en) * 2000-03-01 2005-10-27 Marshall William S Identification and use of effectors and allosteric molecules for the alteration of gene expression
US7514239B2 (en) 2000-03-28 2009-04-07 Amgen Inc. Nucleic acid molecules encoding beta-like glycoprotein hormone polypeptides and heterodimers thereof
US6576202B1 (en) * 2000-04-21 2003-06-10 Kin-Chung Ray Chiu Highly efficient compact capacitance coupled plasma reactor/generator and method
ES2317917T3 (es) 2000-06-28 2009-05-01 Amgen Inc. Moleculas de receptor de linfopoyetina estromal timica y sus usos de las mismas.
AU2001271618B8 (en) * 2000-06-28 2006-04-27 Amgen, Inc. B7-Like molecules and uses thereof
US7399599B2 (en) * 2000-07-10 2008-07-15 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
US7615356B2 (en) * 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
WO2002008748A2 (en) * 2000-07-10 2002-01-31 Aurora Biosciences Corporation Ion channel assay methods
EP1337634A2 (de) 2000-11-28 2003-08-27 Amgen Inc. Neue polypeptide, die an der immunabwehr beteiligt sind
US7029916B2 (en) * 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US7101703B2 (en) * 2001-03-09 2006-09-05 Cornell Research Foundation, Inc. Electrofusion microelectrode
US6562386B2 (en) 2001-05-07 2003-05-13 Regents Of The University Of Minnesota Method and apparatus for non-thermal pasteurization
US6911225B2 (en) * 2001-05-07 2005-06-28 Regents Of The University Of Minnesota Method and apparatus for non-thermal pasteurization of living-mammal-instillable liquids
US7011790B2 (en) * 2001-05-07 2006-03-14 Regents Of The University Of Minnesota Non-thermal disinfection of biological fluids using non-thermal plasma
MXPA03010967A (es) * 2001-05-30 2004-10-28 Chromos Molecular Systems Inc Cromosomas artificiales de plantas, usos de los mismos y metodos para preparar cromosomas artificiales de plantas.
DE10127247B4 (de) * 2001-06-05 2006-12-07 Eppendorf Ag Vorrichtung und Verfahren zur elektrischen Behandlung suspendierter biologischer Partikel
JP2003000225A (ja) * 2001-06-25 2003-01-07 Hakuju Inst For Health Science Co Ltd 細胞培養装置及び細胞培養方法
US20030017587A1 (en) * 2001-07-18 2003-01-23 Rader William C. Embryonic stem cells, clinical applications and methods for expanding in vitro
US20030030374A1 (en) * 2001-08-03 2003-02-13 Deepak Pai Dielectric barrier discharge plasma reactor cell
KR20030012995A (ko) * 2001-08-06 2003-02-14 주식회사 토이랩 Ｄｃ 전원을 사용한 전기천공기
US7141425B2 (en) * 2001-08-22 2006-11-28 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
AU2002254269A1 (en) * 2001-08-31 2003-03-18 Cyto Pulse Sciences, Inc. Non-linear amplitude dielectrophoresis waveform for cell fusion
WO2003026599A1 (en) * 2001-09-26 2003-04-03 The Procter & Gamble Company Personal cleansing compositions comprising silicone resin-containing adhesives
AU2002360540A1 (en) * 2001-12-04 2003-06-17 University Of Southern California Method for intracellular modifications within living cells using pulsed electric fields
US20040025194A1 (en) * 2002-02-22 2004-02-05 Board Of Trustees Of The University Of Illinois Beta chain-associated regulator of apoptosis
US7662924B2 (en) * 2002-02-22 2010-02-16 The Board Of Trustees Of The University Of Illinois Beta chain-associated regulator of apoptosis
US7148059B1 (en) * 2002-02-22 2006-12-12 Ionoptix Corporation Myocyte culture pacing apparatus
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
CN100579577C (zh) * 2002-03-15 2010-01-13 退伍军人事务研发服务部 使用细胞脱唾液酸决定簇和糖缀合物将细胞靶向组织和器官的方法和组合物
US20050233993A1 (en) * 2002-06-05 2005-10-20 Kadonaga James T Methods for promoting homologous recombination
US20040011652A1 (en) * 2002-07-16 2004-01-22 Bressler Vincent Edward Separation of particles using multiple conductive layers
US20040115784A1 (en) * 2002-09-30 2004-06-17 Maxcyte, Inc. Apparatus and method for streaming electroporation
WO2004060911A2 (en) 2002-12-30 2004-07-22 Amgen Inc. Combination therapy with co-stimulatory factors
DE10352416B4 (de) * 2003-11-10 2005-10-20 Evotec Technologies Gmbh Verfahren und Vorrichtungen zur Untersuchung eines deformierbaren Objekts
AU2003290740A1 (en) 2003-12-01 2005-08-12 Richard E. Walters Non-uniform electric field chamber for cell fusion
CA2557485A1 (en) * 2004-02-26 2005-09-09 Thomsen Bioscience A/S Method, chip, device and integrated system for detection biological particles
DK1730519T3 (da) * 2004-02-26 2010-11-01 Delta Dansk Elektronik Lys Og Fremgangsmåde, chip og system til opsamling af biologiske partikler
EP1730276B8 (de) 2004-02-26 2010-07-21 DELTA Dansk Elektronik, Lys og Akustik Verfahren, chip, vorrichtung und system zur extraktion biologischer materialien
CN102268425B (zh) * 2004-05-12 2015-02-25 麦克赛特股份有限公司 与可调流式电穿孔室相关的方法和装置
GB0411610D0 (en) 2004-05-24 2004-06-30 Bioinduction Ltd Electrotherapy apparatus
WO2006009575A1 (en) 2004-06-22 2006-01-26 The Board Of Trustees Of The University Of Illinois METHODS OF INHIBITING TUMOR CELL PROLIFERATION WITH FOXM1 siRNA
US7846720B2 (en) * 2005-01-26 2010-12-07 Vgx Pharmaceuticals, Inc. Optimized high yield synthetic plasmids
US7923251B2 (en) * 2005-02-23 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus for avalanche-mediated transfer of agents into cells
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection
EP1734111B1 (de) 2005-06-13 2020-06-24 Tosoh Corporation Zellfusionsvorrichtung und Verfahren zur Zellfusion unter Verwendung derselben
US20070004025A1 (en) * 2005-06-30 2007-01-04 Junne-Jih Chen Replaceable annular electrode for transgenic
WO2007021993A1 (en) * 2005-08-12 2007-02-22 Maxcyte, Inc. Helical electrode geometries for electroporation
DE102005047131A1 (de) * 2005-09-30 2007-04-12 Evotec Technologies Gmbh Verfahren und Vorrichtung zur Manipulation von sedimentierenden Partikeln
US7915044B2 (en) * 2005-12-22 2011-03-29 Cornell Research Foundation, Inc. Electrofusion microelectrode and methods of using it to manipulate cells and/or cellular components
CN1995361A (zh) * 2006-01-06 2007-07-11 博奥生物有限公司 利用介电电泳辅助细胞定位借以提高电穿孔效率的方法
KR100695272B1 (ko) 2006-01-10 2007-03-14 단국대학교 산학협력단 이종 식물 세포융합장치
US7931811B2 (en) * 2006-10-27 2011-04-26 Regents Of The University Of Minnesota Dielectric barrier reactor having concentrated electric field
US20080305157A1 (en) * 2007-06-08 2008-12-11 University Of Maryland Office Of Technology Commercialization Encapsulation and separation of charged organic solutes inside catanionic vesicles
WO2009018250A2 (en) * 2007-07-30 2009-02-05 University Of Iowa Research Foundation Use of rpa4 and rpa4/rpa32 hybrid polypeptides for modulating cell proliferation
EP2265707A2 (de) * 2008-03-12 2010-12-29 Cellectricon Ab Vorrichtung und verfahren zur spitzenausrichtung bei multiwell-platten
US20090269851A1 (en) * 2008-04-24 2009-10-29 Bio-Rad Laboratories, Inc. A Corporation Of The State Of Delaware Use of disk surface for electroporation of adherent cells
JOP20190083A1 (ar) 2008-06-04 2017-06-16 Amgen Inc بولي ببتيدات اندماجية طافرة لـfgf21 واستخداماتها
EP2154240A1 (de) * 2008-08-06 2010-02-17 UToC B.V. i.o PRF in einer Zell- und Gewebekultur
PE20120021A1 (es) 2008-10-10 2012-02-10 Amgen Inc Mutantes fgf21
FI3248610T3 (fi) 2009-05-05 2024-01-18 Amgen Inc Fgf21-mutantit ja niiden käyttö
MX2011011815A (es) 2009-05-05 2012-01-27 Amgen Inc Mutantes fgf21 y usos de los mismos.
EP2443145A1 (de) 2009-06-17 2012-04-25 Amgen, Inc Chimäre fgf19-polypeptide und ihreverwendung
IT1395992B1 (it) * 2009-10-27 2012-11-09 Caccia Testa per elettroporazione
CN102712933A (zh) 2009-11-05 2012-10-03 西马生物医学计划公司 调控的表达系统
EP2506861A1 (de) * 2009-12-02 2012-10-10 Amgen Inc. Bindeproteine zur bindung von humanem fgfr1c, humanem b-klotho sowie humanem fgfr1c als auch humanem b-klotho
UA109888C2 (uk) 2009-12-07 2015-10-26 ІЗОЛЬОВАНЕ АНТИТІЛО АБО ЙОГО ФРАГМЕНТ, ЩО ЗВ'ЯЗУЄТЬСЯ З β-КЛОТО, РЕЦЕПТОРАМИ FGF І ЇХНІМИ КОМПЛЕКСАМИ
JP2013523184A (ja) 2010-04-15 2013-06-17 アムジエン・インコーポレーテツド ヒトＦＧＦ受容体およびβ−ＫＬＯＴＨＯ結合性タンパク質
CA2800065A1 (en) 2010-05-21 2011-11-24 Peptimed, Inc. Reagents and methods for treating cancer
US10131900B2 (en) * 2010-12-15 2018-11-20 Old Dominion University Research Foundation Electroporation-induced electrosensitization
WO2012095548A2 (es) 2011-01-13 2012-07-19 Centro De Investigación Biomédica En Red De Enfermedades Neurodegenerativas (Ciberned) Compuestos para el tratamiento de enfermedades neurodegenerativas
US11198126B2 (en) * 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
JOP20140087B1 (ar) 2013-03-13 2021-08-17 Amgen Inc بروتينات مخصصة ل baff و b7rp1 وإستخداماتها
US9458246B2 (en) 2013-03-13 2016-10-04 Amgen Inc. Proteins specific for BAFF and B7RP1
DE202013009847U1 (de) * 2013-12-06 2015-03-09 Hugo Vogelsang Maschinenbau Gmbh Vorrichtung zur elektrischen Desintegration mit mehreren Wirkzonen
US9739751B2 (en) 2015-02-05 2017-08-22 International Business Machines Corporation Devices for trapping and controlling microparticles with radiation
EP3442711B1 (de) 2016-04-15 2020-12-09 Fluid-Screen, Inc. Analytnachweisverfahren und -vorrichtungen mit dielektrophorese und elektroosmose
US10183058B2 (en) 2016-06-17 2019-01-22 Nymox Corporation Method of preventing or reducing the progression of prostate cancer
US10172910B2 (en) 2016-07-28 2019-01-08 Nymox Corporation Method of preventing or reducing the incidence of acute urinary retention
US10532081B2 (en) 2016-09-07 2020-01-14 Nymox Corporation Method of ameliorating or preventing the worsening or the progression of symptoms of BPH
EP3559228A4 (de) 2016-11-09 2021-01-27 Sigma Genetics, Inc. Systeme, vorrichtungen und verfahren zur durch magnetfelder induzierten elektroporation
WO2019108957A1 (en) * 2017-12-01 2019-06-06 Alxerion Biotech Corp. Device and system for loaded cellular vesicles and uses thereof
WO2021097035A1 (en) 2019-11-13 2021-05-20 Fluid-Screen, Inc. An apparatus and methods to rapidly detect, separate, purify, and quantify various viruses from cells, cultured medium and other fluids
CA3161454A1 (en) 2019-11-13 2021-05-20 Fluid-Screen, Inc. Methods and apparatus for detection of bacteria in a sample using dielectrophoresis
US11191977B1 (en) 2020-11-12 2021-12-07 Howard Letovsky Applications of bioactive frequencies and menus thereof
EP4053260A1 (de) * 2021-03-04 2022-09-07 Bühler AG Vorrichtung mit einem einsatz zur behandlung von zellmaterial

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3095359A (en) * 1959-11-16 1963-06-25 New England Inst For Medical R High-frequency treatment of matter
US4849355A (en) * 1985-01-08 1989-07-18 Wong Tai Kin Method of transferring genes into cells
US4452747A (en) * 1982-03-22 1984-06-05 Klaus Gersonde Method of and arrangement for producing lipid vesicles
US4578167A (en) * 1982-09-28 1986-03-25 Biofusion, Inc. Cell fusion
US4476004A (en) * 1983-04-08 1984-10-09 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
US4441972A (en) * 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
DE3317415A1 (de) * 1983-05-13 1984-11-15 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur behandlung von zellen im elektrischen feld
DE3321226C2 (de) * 1983-06-11 1985-05-09 Kernforschungsanlage Jülich GmbH, 5170 Jülich Verfahren zur Fusion von Zellen
US4524079A (en) * 1983-11-10 1985-06-18 Maxwell Laboratories, Inc. Deactivation of microorganisms by an oscillating magnetic field
US4661451A (en) * 1984-02-06 1987-04-28 Ortho Diagnostic Systems, Inc. Methods for immobilizing and translocating biological cells
US4665898A (en) * 1984-05-23 1987-05-19 Maxwell Laboratories, Inc. Malignancy treatment
US4578168A (en) * 1984-07-27 1986-03-25 Biotronics Apparatus for fusing live cells with electric fields
US4561961A (en) * 1984-07-27 1985-12-31 Biotronics Cooled microscope slide and electrode apparatus for use in live cell fusion system
US4622302A (en) * 1984-08-09 1986-11-11 American National Red Cross Process for inducing membrane fusion under an electric field
DE3437470A1 (de) * 1984-10-12 1986-04-17 GCA Corp., Bedford, Mass. Medium zur herstellung von lebensfaehigen, fusionierten zellen
US4695547A (en) * 1986-04-02 1987-09-22 Jeffrey L. Hilliard Probe for electrofusion, electroporation, or like procedure
US4906576A (en) * 1986-05-09 1990-03-06 Electropore, Inc. High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor
US5128257A (en) * 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
US4822470A (en) * 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202013005125U1 (de) * 2013-06-04 2014-09-05 Hugo Vogelsang Maschinenbau Gmbh Vorrichtung zur elektrischen Desintegration von Zellverbänden
DE102018105931A1 (de) * 2018-03-14 2019-09-19 Technische Universität Darmstadt Mikrowellenporator

Also Published As

Publication number Publication date
AU2787989A (en) 1989-05-02
US4970154A (en) 1990-11-13
DE3856549D1 (de) 2003-01-30
US5304486A (en) 1994-04-19
EP0710718B1 (de) 2002-12-18
DE3855330T2 (de) 1996-11-21
DE3855330D1 (de) 1996-07-04
EP0710718A1 (de) 1996-05-08

Similar Documents

Publication Publication Date Title
DE3856549T2 (de) Apparat zur Zellpermeabilisierung und Zellfusion unter Verwendung elektrischer Radiofrequenz-Impulse
IL87932A0 (en) Method and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
EG18146A (en) Method and apparatus for refining glassy material
DK451187D0 (da) Fremgangsmaade og apparat til kraftproduktion
JPS5765180A (en) Method and apparatus for culturing cell
DE3568999D1 (en) Method and apparatus for incubating cells
GB2119184B (en) High frequency current supply method and apparatus
EP0204448A3 (en) Method of and apparatus for spin-welding
EP0411658A3 (en) Method for culturing cells
EP0419234A3 (en) Cell culture apparatus
NO865206D0 (no) Fremgangsmaate og apparat for fremstilling av skummende termoplastgjenstander.
NO175244C (no) Fremgangsmåte og apparat for å tilvirke deler med utgangspunkt i pulvermateriale
DK76088D0 (da) Fremgangsmaade og apparat for frembringelse af et foreskrevet elektrisk signal
DK394687A (da) Freemgangsmaade og apparat til styring af en inverter
EP0269086A3 (en) Cell culture apparatus
IL83853A (en) Open cell ceramic foams and method for their preparation
DE3568798D1 (en) Cell and tissue culture process and apparatus
IL82782A (en) Method and apparatus for the electric melting of glass
EP0300588A3 (en) Cell culturing apparatus and method
GB8917911D0 (en) Cell culture apparatus
EP0340783A3 (en) Apparatus for culturing animal cells, method of culturing thereof and diagnostics of the culture
EP0219791A3 (en) Process for culturing recombinant cells
GB2189072B (en) Method and apparatus for sizing galvanic cell separators
GB8821638D0 (en) Cell screening apparatus & methods
IL70840A0 (en) Cell culture apparatus and process using an immobilized cell composite

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee