CA2203886A1 - Patch clamp apparatus and technique having high throughput and low fluid volume requirements - Google Patents

Patch clamp apparatus and technique having high throughput and low fluid volume requirements

Info

Publication number
CA2203886A1
CA2203886A1 CA002203886A CA2203886A CA2203886A1 CA 2203886 A1 CA2203886 A1 CA 2203886A1 CA 002203886 A CA002203886 A CA 002203886A CA 2203886 A CA2203886 A CA 2203886A CA 2203886 A1 CA2203886 A1 CA 2203886A1
Authority
CA
Canada
Prior art keywords
high throughput
patch clamp
utilizing
novel
tested
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002203886A
Other languages
French (fr)
Other versions
CA2203886C (en
Inventor
Soren-Peter Olesen
Palle Christophersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sophion Bioscience AS
Original Assignee
Soren-Peter Olesen
Palle Christophersen
Neurosearch A/S
Sophion Bioscience A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Soren-Peter Olesen, Palle Christophersen, Neurosearch A/S, Sophion Bioscience A/S filed Critical Soren-Peter Olesen
Publication of CA2203886A1 publication Critical patent/CA2203886A1/en
Application granted granted Critical
Publication of CA2203886C publication Critical patent/CA2203886C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Abstract

Apparatus for carrying out patch clamp technique utilized in studying the effect of certain materials on ion tranfer channels in biological tissue, and more particularly patch clamp apparatus utilizing an autosampler, such as those utilized with HPLC apparatus, to provide high throughput, is disclosed.
The invention additionally includes novel microperfusion chamber assemblies capable of utilizing only small amounts of material to be tested and only small amounts of liquid carrier, thereby enabling many tests to be completed in a short period of time. The invention more broadly relates to a novel electrophysiology drug handling and application set up for screening of chemical substances while providing high throughput and requiring only low volume of solutions and samples to be tested. The invention also comprises several novel procedures for utilizing the apparatus of the invention.
CA002203886A 1994-10-28 1995-06-07 Patch clamp apparatus and technique having high throughput and low fluid volume requirements Expired - Fee Related CA2203886C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK125294 1994-10-28
DK1252/94 1994-10-28
PCT/EP1995/002204 WO1996013721A1 (en) 1994-10-28 1995-06-07 Patch clamp apparatus and technique having high throughput and low fluid volume requirements

Publications (2)

Publication Number Publication Date
CA2203886A1 true CA2203886A1 (en) 1996-05-09
CA2203886C CA2203886C (en) 2003-09-02

Family

ID=8102743

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002203886A Expired - Fee Related CA2203886C (en) 1994-10-28 1995-06-07 Patch clamp apparatus and technique having high throughput and low fluid volume requirements

Country Status (12)

Country Link
US (2) US6063260A (en)
EP (1) EP0788600B1 (en)
JP (1) JP3688713B2 (en)
AT (1) ATE216080T1 (en)
AU (1) AU2788695A (en)
CA (1) CA2203886C (en)
DE (1) DE69526364T2 (en)
DK (1) DK0788600T3 (en)
ES (1) ES2171542T3 (en)
PT (1) PT788600E (en)
SI (1) SI0788600T1 (en)
WO (1) WO1996013721A1 (en)

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US20020035459A1 (en) 1998-09-14 2002-03-21 George M. Grass Pharmacokinetic-based drug design tool and method
KR100736059B1 (en) 1998-12-05 2007-07-06 젠션 디스커버리 리미티드 Interface patch clamping
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US6488829B1 (en) 1999-08-05 2002-12-03 Essen Instruments Inc High-throughput electrophysiological measurement apparatus
DE10008373C2 (en) * 2000-02-23 2002-11-28 Helmut Adelsberger Method and device for determining ion channel activity
US20030139336A1 (en) * 2000-03-21 2003-07-24 Norwood James Henry Interface patch clamping
US6541243B1 (en) * 2000-06-05 2003-04-01 Axon Instruments, Inc. Perfusion chamber for electrophysiological testing of oocytes
US20030129581A1 (en) * 2000-06-06 2003-07-10 Owen David Geraint Patch-clamping method and apparatus
GB0013584D0 (en) * 2000-06-06 2000-07-26 Cenes Ltd Automated flow patch-clamp system
US6686193B2 (en) 2000-07-10 2004-02-03 Vertex Pharmaceuticals, Inc. High throughput method and system for screening candidate compounds for activity against target ion channels
US7615356B2 (en) * 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
US7067046B2 (en) * 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
US7270730B2 (en) * 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
WO2002059597A1 (en) * 2001-01-26 2002-08-01 Cytion Sa Methods and devices for the electrically tight adhesion of a cell to a surface
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US6815197B2 (en) * 2001-02-16 2004-11-09 Multi Channel System Mcs Gmbh Apparatus for conducting electrophysiological measurements on cells
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US20060029955A1 (en) * 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
CA2441366A1 (en) * 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20050058990A1 (en) * 2001-03-24 2005-03-17 Antonio Guia Biochip devices for ion transport measurement, methods of manufacture, and methods of use
US6969489B2 (en) 2001-08-24 2005-11-29 Cytoplex Biosciences Micro array for high throughout screening
WO2003021230A2 (en) * 2001-09-05 2003-03-13 Essen Instruments, Inc. High-throughput electrophysiological measurement system
AU2002352988A1 (en) * 2001-11-30 2003-06-17 Bristol-Myers Squibb Company Pipette configurations and arrays thereof for measuring cellular electrical properties
WO2003047738A2 (en) * 2001-11-30 2003-06-12 Bristol-Myers Squibb Company Liquid interface configurations for automated patch clamp recording
DE10202887B4 (en) * 2002-01-25 2004-05-06 Advalytix Ag Cell analysis method
AU2003205132A1 (en) * 2002-02-12 2003-09-04 Celectricon AB Systems and methods for rapidly changing the solution environment around sensors
AU2003226075B2 (en) * 2002-04-12 2008-09-25 Instrumentation Laboratory Company Immunoassay probe
EP1501924A4 (en) * 2002-05-04 2006-05-24 Aviva Biosciences Corp Apparatus including ion transport detecting structures and methods of use
US20030215815A1 (en) * 2002-05-20 2003-11-20 Clark William G. Screening method
US20030215872A1 (en) * 2002-05-20 2003-11-20 Clark-Mxr, Inc. Screening apparatus and method for making
US8202439B2 (en) * 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
JP4552423B2 (en) * 2003-11-21 2010-09-29 パナソニック株式会社 Extracellular potential measuring device and method for measuring extracellular potential using the same
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US7465558B2 (en) * 2004-05-12 2008-12-16 Wyeth Perfusion system and apparatus for automated multi-channel patch-clamp recordings utilizing inside-out whole-cell configuration
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PL2404919T3 (en) 2005-11-08 2014-01-31 Vertex Pharma Heterocyclic compound useful as a modulator of ATP-binding cassette transporters.
ES2604480T5 (en) 2010-04-07 2020-03-17 Vertex Pharma 3- (6- (1- (2,2-Difluorobenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid pharmaceutical compositions and administration thereof
US9086401B2 (en) * 2013-03-06 2015-07-21 Wisconsin Alumni Research Foundation Radio-frequency ion channel antenna
RU2691136C2 (en) * 2014-11-18 2019-06-11 Вертекс Фармасьютикалз Инкорпорейтед High-performance test high-performance liquid chromatography method
US10479972B2 (en) * 2015-04-20 2019-11-19 Rush University Medical Center Chamber for imaging cells and method of use thereof
US10324080B2 (en) * 2015-11-17 2019-06-18 Purdue Research Foundation Systems and methods for automated image-guided patch-clamp electrophysiology in vitro
EP4209783A1 (en) 2016-12-02 2023-07-12 Sophion Bioscience A/S Seal enhancer
US11073508B2 (en) 2017-03-28 2021-07-27 Arizona Board Of Regents On Behalf Of The University Of Arizona Rapid conductance based ion channel analysis
WO2022214558A1 (en) * 2021-04-07 2022-10-13 Norgren LLC Bioprocessing systems

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Also Published As

Publication number Publication date
DK0788600T3 (en) 2002-08-05
EP0788600B1 (en) 2002-04-10
ATE216080T1 (en) 2002-04-15
EP0788600A1 (en) 1997-08-13
PT788600E (en) 2002-07-31
JPH10509794A (en) 1998-09-22
ES2171542T3 (en) 2002-09-16
WO1996013721A1 (en) 1996-05-09
DE69526364T2 (en) 2002-11-28
DE69526364D1 (en) 2002-05-16
SI0788600T1 (en) 2002-06-30
US6063260A (en) 2000-05-16
US6117291A (en) 2000-09-12
JP3688713B2 (en) 2005-08-31
AU2788695A (en) 1996-05-23
CA2203886C (en) 2003-09-02

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